Detection of human coronaviruses in children with acute gastroenteritis
Identifieur interne : 002944 ( Main/Exploration ); précédent : 002943; suivant : 002945Detection of human coronaviruses in children with acute gastroenteritis
Auteurs : Minna Risku [Finlande] ; Suvi Lappalainen [Finlande] ; Sirpa R S Nen [Finlande] ; Timo Vesikari [Finlande]Source :
- Journal of clinical virology [ 1386-6532 ] ; 2010.
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- Pascal (Inist)
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English descriptors
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Abstract
Background: Human coronaviruses (HCoVs) are known respiratory pathogens. Moreover, coronavirus-like particles have been seen by electron microscope in stools, and SARS-HCoV has been isolated from intestinal tissue and detected in stool samples. Objectives: To find out if HCoVs can be found in stools of children with acute gastroenteritis and to assess the significance of HCoVs in the etiology of acute gastroenteritis in children. Study design: 878 stool specimens from children with acute gastroenteritis and 112 from control children were tested by RT-PCR to detect HCoV groups 1 B, 2A and SARS. HCoVs were typed by sequencing all PCR positive samples. Results: Twenty-two (2.5%) of the 878 stool specimens of children with acute gastroenteritis were positive for HCoVs. The following HCoV types were detected: OC43 (10 cases, 45.5%), HKU1 (6 cases, 27.3%), 229E (2 cases, 9.1%) and NL63 (4 cases, 18.2%). In 4 of the cases a HCoV was the only detected virus; in the remaining cases rotavirus or norovirus was found in the same sample. In control groups there were two HCoV positive samples of 112 tested. Conclusions: This study shows that all known non-SARS HCoVs can be found in stools of children with acute gastroenteritis. On the basis of this study, the significance of coronaviruses as gastrointestinal pathogens in children appears minor, since most of the coronavirus findings were co-infections with known gastroenteritis viruses.
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Suvi" uniqKey="Lappalainen S" first="Suvi" last="Lappalainen">Suvi Lappalainen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Medical School, Biokatu 10, University of Tampere</s1><s2>33014 Tampere</s2><s3>FIN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Finlande</country><wicri:noRegion>33014 Tampere</wicri:noRegion></affiliation></author><author><name sortKey="R S Nen, Sirpa" sort="R S Nen, Sirpa" uniqKey="R S Nen S" first="Sirpa" last="R S Nen">Sirpa R S Nen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Medical School, Biokatu 10, University of Tampere</s1><s2>33014 Tampere</s2><s3>FIN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Finlande</country><wicri:noRegion>33014 Tampere</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Pediatrics, Tampere University 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level="j" type="abbreviated">J. clin. virol.</title><idno type="ISSN">1386-6532</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical virology</title><title level="j" type="abbreviated">J. clin. virol.</title><idno type="ISSN">1386-6532</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term><term>Child</term><term>Coronavirus</term><term>Detection</term><term>Gastroenteritis</term><term>Human</term><term>Reverse transcription polymerase chain reaction</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Homme</term><term>Coronavirus</term><term>Détection</term><term>Enfant</term><term>Aigu</term><term>Réaction chaîne polymérase RT</term><term>Gastroentérite</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Enfant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: Human coronaviruses (HCoVs) are known respiratory pathogens. Moreover, coronavirus-like particles have been seen by electron microscope in stools, and SARS-HCoV has been isolated from intestinal tissue and detected in stool samples. Objectives: To find out if HCoVs can be found in stools of children with acute gastroenteritis and to assess the significance of HCoVs in the etiology of acute gastroenteritis in children. Study design: 878 stool specimens from children with acute gastroenteritis and 112 from control children were tested by RT-PCR to detect HCoV groups 1 B, 2A and SARS. HCoVs were typed by sequencing all PCR positive samples. Results: Twenty-two (2.5%) of the 878 stool specimens of children with acute gastroenteritis were positive for HCoVs. The following HCoV types were detected: OC43 (10 cases, 45.5%), HKU1 (6 cases, 27.3%), 229E (2 cases, 9.1%) and NL63 (4 cases, 18.2%). In 4 of the cases a HCoV was the only detected virus; in the remaining cases rotavirus or norovirus was found in the same sample. In control groups there were two HCoV positive samples of 112 tested. Conclusions: This study shows that all known non-SARS HCoVs can be found in stools of children with acute gastroenteritis. On the basis of this study, the significance of coronaviruses as gastrointestinal pathogens in children appears minor, since most of the coronavirus findings were co-infections with known gastroenteritis viruses.</div></front></TEI><affiliations><list><country><li>Finlande</li></country></list><tree><country name="Finlande"><noRegion><name sortKey="Risku, Minna" sort="Risku, Minna" uniqKey="Risku M" first="Minna" last="Risku">Minna Risku</name></noRegion><name sortKey="Lappalainen, Suvi" sort="Lappalainen, Suvi" uniqKey="Lappalainen S" first="Suvi" last="Lappalainen">Suvi Lappalainen</name><name sortKey="R S Nen, Sirpa" sort="R S Nen, Sirpa" uniqKey="R S Nen S" first="Sirpa" last="R S Nen">Sirpa R S Nen</name><name sortKey="R S Nen, Sirpa" sort="R S Nen, Sirpa" uniqKey="R S Nen S" first="Sirpa" last="R S Nen">Sirpa R S Nen</name><name sortKey="Vesikari, Timo" sort="Vesikari, Timo" uniqKey="Vesikari T" first="Timo" last="Vesikari">Timo Vesikari</name><name sortKey="Vesikari, Timo" sort="Vesikari, Timo" uniqKey="Vesikari T" first="Timo" last="Vesikari">Timo Vesikari</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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